| alpha-Tocopherol-TEG Phosphoramidite |
Catalog Number: 10-1977-xx
Description: α-Tocopherol-TEG Phosphoramidite
1-Dimethoxytrityloxy-3-O-[(9-DL-α-tocopheryl)-triethyleneglycol-1-yl]-
glyceryl-2-O-[(2-cyanoethyl)-(N,N,-diisopropyl)]-phosphoramidite |
| Formula: C68H103N2O10P |
M.W.: 1139.56 |
F.W.: 698.91 |
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
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| Coupling: 15 minute coupling time. DCI activator (30-3150-xx) is recommended. To maintain label yield, carry out the synthesis DMT-ON. |
| Deprotection: To maintain label yield as a 5' modifier, treat column with 10% diethylamine in acetonitrile for 2 minutes at room temperature (2x) to remove cyanoethyl protecting groups. Rinse column with ACN and then cleave and deprotect as required by nucleobases. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
TOCOPHEROL LABELLING
Vitamin E is both lipophilic and non-toxic even at high doses so would be an excellent candidate as a lipophilic carrier for oligonucleotides. Therefore, as an addition to our cholesteryl product line, we offer simple a-tocopheryl (vitamin E) labelling. Totally synthetic a-tocopherol is racemic at its three chiral centers and is used to prepare this product.
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