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Oligo Synthesis

Oligo Synthesis : CEPs

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2',3'-ddA-CE Phosphoramidite

2',3'-ddA-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-7001-022',3'-ddA-CE Phosphoramidite 0.25g £573.00 Quantity Add to Order
10-7001-902',3'-ddA-CE Phosphoramidite 100µmoles £136.00 Quantity Add to Order

Description

2',3'-ddA-CE Phosphoramidite

Structure

Catalog Number: 10-7001-xx

Description: 2',3'-ddA-CE Phosphoramidite

N6-diisobutylaminomethylidene-2',3'-dideoxyAdenosine,5'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C28H47N8O3P M.W.: 574.70 F.W.: 297.21
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: 17 hours in Ammonium Hydroxide at 55°C or 1 hour at 65°C in Ammonium Hydroxide/40% Methylamine 1:1 (v/v) (AMA).
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

chain terminators

In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)

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Protocols

MSDS

Glen Report

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Applications & Benefits

To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective.  2’,3’-phosphoramidites are designed to be used with the 5’-phosphoramidites and supports.  Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique.  Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed.  (3’-termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-7001-02 0.25grams .25grams 4.35 131.67 79 49.38 35.91 26.33 6.58
10-7001-90 100µmoles .057grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-7001-02 0.25grams .25grams 6.49 .07 123.4 77.13 56.09 7.71  
10-7001-90 100µmoles .057grams 1.5 .07 23.6 14.75 10.73 1.48  
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-7001-02 0.25grams .25grams 6.49 .07 125 78.13 56.82    
10-7001-90 100µmoles .057grams 1.5 .07 25.2 15.75 11.45

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Related products

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