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Oligo Synthesis

Oligo Synthesis : CEPs

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2'-OMe-G-CE Phosphoramidite

2'-OMe-G-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3121-022'-OMe-G-CE Phosphoramidite 0.25g £45.00 Quantity Add to Order
10-3121-052'-OMe-G-CE Phosphoramidite 0.5g £91.00 Quantity Add to Order
10-3121-102'-OMe-G-CE Phosphoramidite 1.0g £182.00 Quantity Add to Order
10-3121-902'-OMe-G-CE Phosphoramidite 100µmoles £18.00 Quantity Add to Order

Description

2'-OMe-G-CE Phosphoramidite

2’-OMe-RNA phosphoramidites

Glen Research 2’-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2’-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2’-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

Structure

Catalog Number: 10-3121-xx

Description: 2'-OMe-G-CE Phosphoramidite

5'-Dimethoxytrityl-N-dimethylaminomethylidene-Guanosine,2'-O-methyl,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C44H55N8O8P M.W.: 854.93 F.W.: 359.24
Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time recommended
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 3-5 days

2’-Ome-rna phosphoramidites

Glen Research 2’-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2’-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2’-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

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Protocols

MSDS

Glen Report 6.2

Glen Report 6.1

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References

(1) B. Oberhauser and E. Wagner, Nucleic Acids Res., 1992, 20, 533-538.
(2) J. Goodchild, Nucleic Acids Res., 1992, 20, 4607-4612.
(3) C. Giovannangeli, N.T. Thuong, and C. Helene, Nucleic Acids Res., 1992, 20, 4275-4281.
(4) C. Giovannangeli, M. Rougee, T. Garestier, N.T. Thuong, and C. Helene, Proc. Natl. Acad. Sci. U.S.A., 1992, 89, 8631-8635.
(5) J.A. Doudna, J.W. Szostak, A. Rich, and N. Usman, J. Org. Chem., 1990, 55, 5547-5549.

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Notes

2'-OMe-G-CE Phosphoramidite

5'-Dimethoxytrityl-N-dimethylaminomethylidene-Guanosine,2'-O-methyl,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C44H55N8O8P M.W.: 854.93 F.W.: 359.24
Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time recommended
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 3-5 days
  Material Safety Data Sheet

Literature Highlights

Glen Report 6.2: RESEARCH REVIEW - ANTISENSE RNA
Glen Report 6.1: 2'-OMe-RNA UPDATE

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3121-02 0.25grams .25grams 2.92 84 50.4 31.5 22.91 16.8 4.2
10-3121-05 0.5grams .5grams 5.85 181.67 109 68.13 49.55 36.33 9.08
10-3121-10 1.0gram 1grams 11.7 376.67 226 141.25 102.73 75.33 18.83
10-3121-90 100µmoles .085grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-3121-02 0.25grams .25grams 4.36 .067 80.8 50.5 36.73 5.05  
10-3121-05 0.5grams .5grams 8.73 .067 168.2 105.13 76.45 10.51  
10-3121-10 1.0gram 1grams 17.46 .067 342.8 214.25 155.82 21.43  
10-3121-90 100µmoles .085grams 1.5 .067 23.6 14.75 10.73 1.48  
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-3121-02 0.25grams .25grams 4.36 .067 82.4 51.5 37.45    
10-3121-05 0.5grams .5grams 8.73 .067 169.8 106.13 77.18    
10-3121-10 1.0gram 1grams 17.46 .067 344.4 215.25 156.55    
10-3121-90 100µmoles .085grams 1.5 .067 25.2 15.75 11.45

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3121-02 0.25grams .25grams 2.92 84 50.4 31.5 22.91 16.8 4.2
10-3121-05 0.5grams .5grams 5.85 181.67 109 68.13 49.55 36.33 9.08
10-3121-10 1.0gram 1grams 11.7 376.67 226 141.25 102.73 75.33 18.83
10-3121-90 100µmoles .085grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3121-02 0.25grams .25grams 4.36 .07 80.8 50.5 36.73 5.05
10-3121-05 0.5grams .5grams 8.73 .07 168.2 105.13 76.45 10.51
10-3121-10 1.0gram 1grams 17.46 .07 342.8 214.25 155.82 21.43
10-3121-90 100µmoles .085grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3121-02 0.25grams .25grams 4.36 .07 82.4 51.5 37.45

10-3121-05 0.5grams .5grams 8.73 .07 169.8 106.13 77.18

10-3121-10 1.0gram 1grams 17.46 .07 344.4 215.25 156.55

10-3121-90 100µmoles .085grams 1.5 .07 25.2 15.75 11.45

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Related products

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