Catalog Number: 20-2955-xx
Description: 3'-BiotinTEG CPG
1-Dimethoxytrityloxy-3-O-(N-biotinyl-3-aminopropyl)-triethyleneglycolyl-
glyceryl-2-O-succinyl-long chain alkylamino-CPG |
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F.W.: 569.61 |
Diluent: Not Applicable |
| Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. |
| Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: Not Applicable |
Biotin-dT can replace dT residues within the oligonucleotide sequence. 5’-Biotin phosphoramidite can be added ONLY ONCE to the 5’-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques. PC Biotin is a photocleavable 5’-biotin phosphoramidite. BiotinTEG CPG and Protected BiotinLC Serinol CPG are designed for the direct synthesis of oligonucleotides containing biotin at the 3’ terminus.
Desthiobiotin is a biotin analogue that exhibits lower binding to biotin-binding proteins such as streptavidin. This biotin analogue is lacking the sulfur group from the molecule and has a dissociation constant (Kd) several orders of magnitude less than biotin/streptavidin. As a result, biomolecules containing desthiobiotin are dissociated from streptavidin simply in the presence of buffered solutions of biotin. We offer desthiobiotinTEG phosphoramidite and the corresponding CPG.
ABI-style vials and columns are supplied unless otherwise requested (see note box).
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