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Oligo Synthesis

Oligo Synthesis : CEPs

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3'-dA-CE Phosphoramidite

3'-dA-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1004-023'-dA-CE Phosphoramidite 0.25g £1,200.00 Quantity Add to Order
10-1004-903'-dA-CE Phosphoramidite 100µmoles £432.00 Quantity Add to Order
10-1004-953'-dA-CE Phosphoramidite 50µmoles £227.00 Quantity Add to Order

Description

3'-dA-CE Phosphoramidite

Structure

Catalog Number: 10-1004-xx

Description: 3'-dA-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-3'-deoxyAdenosine,2'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C47H52N7O7P M.W.: 857.95 F.W.: 313.21
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites

BIOLOGICAL MIMICS

Our product, 5’-I-dT-CE Phosphoramidite, has been moved to the click chemistry section of this WEB site and and 2,4-Difluorotoluene (F), as a non-polar mimic of thymidine, has been discontinued.

 

See Also

5’-I-dT in Click Chemistry

2’-5’ Linked Oligonucleotides

Cellular DNA and RNA are made up of ribo- and 2’-deoxyribonucleic acids linked together via 3’-5’ phosphodiester linkages and by far comprise the bulk of polynucleic acids found in cells. Much less common are oligonucleotides which have 2’-5’ linkages. However, a unique feature of 2’-5’ linked oligonucleotides is their ability to bind selectively to complementary RNA. These features suggest a number of interesting uses for 2’-5’ linked oligos such as their use as RNA specific probes or in antisense oligos. Recently, oligos have been synthesized using 3’-deoxy-2’-phosphoramidites and 2’-deoxy-3’-phosphoramidites to produce chimeras with 2’-5’ linked ends and 3’-5’ linked central regions. It was found that 2’-5’ phosphorothioate oligos: 1) bind selectively to complementary RNA with the same affinity as phosphodiester oligos; 2) exhibit much nonspecific binding to cellular proteins; 3) do not activate RNase H. A 3’-deoxynucleoside at the 3’-terminus of an otherwise normal oligonucleotide effectively blocks polymerase extension.

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Protocols

MSDS

Glen Repoort 10.1

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1004-02 0.25grams .25grams 2.91 83.67 50.2 31.38 22.82 16.73 4.18
10-1004-90 100µmoles .086grams 1 20 12 7.5 5.45 4 1
10-1004-95 50µmoles .043grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1004-02 0.25grams .25grams 4.35 .07 80.6 50.38 36.64 5.04
10-1004-90 100µmoles .086grams 1.5 .07 23.6 14.75 10.73 1.48
10-1004-95 50µmoles .043grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1004-02 0.25grams .25grams 4.35 .07 82.2 51.38 37.36

10-1004-90 100µmoles .086grams 1.5 .07 25.2 15.75 11.45

10-1004-95 50µmoles .043grams .75 .07 10.2 6.38 4.64

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Related products

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