Catalog Number: 20-2902-xx
Description: 3'-Glyceryl CPG
3-[(4,4'-Dimethoxytrityoxy)glyceryl-1-succinoyl]-
long chain alkylamino-CPG |
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F.W.: 154.06 |
Diluent: Not Applicable |
| Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. |
| Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
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Stability in Solution: Not Applicable
The 3’-Thiol-Modifier S-S CPG supports are used to introduce 3’-thiol linkages with three and six carbon spacers in oligonucleotides. DTPA CPG is used to introduce a dithiol group at the 3’-terminus. In conjunction with DTPA Phosphoramidite, it is simple to produce oligonucleotides with multiple thiol groups at the 3’ terminus, which is ideal for conjugation to gold surfaces. With Glyceryl CPG the 3’-terminus of an oligonucleotide is readily oxidized by sodium periodate to form a 3’-phosphoglycaldehyde. The aldehyde may be further oxidized to the corresponding carboxylic acid. Either the aldehyde or the carboxylate may be used for subsequent conjugation to amine-containing products.
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