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Oligo Synthesis

Oligo Synthesis : Supports, CPGs

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3'-Spacer C3 CPG

3'-Spacer C3 CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
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20-2913-013'-Spacer C3 CPG 0.1g €76.80 Quantity Add to Order
20-2913-103'-Spacer C3 CPG 1.0g €523.20 Quantity Add to Order
20-2913-133'-Spacer C3 CPG 1x10µm €196.80 Quantity Add to Order
20-2913-143'-Spacer C3 CPG 1x15µm €306.00 Quantity Add to Order
20-2913-413'-Spacer C3 CPG 4x1.0µm €109.20 Quantity Add to Order
20-2913-423'-Spacer C3 CPG 4x0.2µm €66.00 Quantity Add to Order

Description

3'-Spacer C3 CPG

 

Structure

Catalog Number: 20-2913-xx

Description: 3'-Spacer C3 CPG

(1-Dimethoxytrityloxy-propanediol-3-succinoyl)-long chain alkylamino-CPG


F.W.: 138.06
Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: Not Applicable

SPACER MODIFIERS

The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3’-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3’-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers

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Protocols

MSDS 

Glen Report 8.1: 3'-TERMINATORS

Glen Report 6.2: RESEARCH REVIEW - ANTISENSE RNA

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Notes

QUESTION: Please send me more information regarding the 3' spacer columns that will protect against exonuclease and polymerase activity?

RESPONSE:We have no definitive data on the propyl CPG to support the assertion that it protects oligos from exonuclease digestion and does not permit polymerase extension. Our conclusion is based by analogy to the propylamino-modifier CPG(1) (Cat. No. 20-2950-41). This modification protects oligos from exonuclease digestion but permits polymerase extension to a small extent since the modifier is eliminated to a level of about 10% from the 3' terminus, leaving the 3'-hydroxyl group available. HPLC experiments have shown that there is no detectable elimination of the propyl group from oligos made from the spacer C3-CPG.

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Related products

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