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Oligo Synthesis

Oligo Synthesis : CEPs

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5-Hydroxymethyl-dU-CE Phosphoramidite

5-Hydroxymethyl-dU-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1093-025-Hydroxymethyl-dU-CE Phosphoramidite 0.25g £777.00 Quantity Add to Order
10-1093-905-Hydroxymethyl-dU-CE Phosphoramidite 100µmoles £255.00 Quantity Add to Order

Description

5-Hydroxymethyl-dU-CE Phosphoramidite
 Structure

DNA Damage/Repair

Cellular DNA is constantly being damaged by oxidation and alkylation, by free radicals, and by ultraviolet and ionizing radiation. The body has therefore evolved a number of repair enzyme systems to excise and repair these lesions. The 8-oxo purine monomers allow investigation of the structure and activity of oligonucleotides containing an 8-oxo mutation which is formed naturally when DNA is subjected to oxidative conditions or ionizing radiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that are structural components of alanine transfer RNA. Dihydrouracil and the hydroxy pyrimidines are major base damage products formed by exposure of DNA to ionizing radiation.

DNA Damage/Repair

Cellular DNA is constantly being damaged by oxidation and alkylation, by free radicals, and by ultraviolet and ionizing radiation. The body has therefore evolved a number of repair enzyme systems to excise and repair these lesions. The 8-oxo purine monomers allow investigation of the structure and activity of oligonucleotides containing an 8-oxo mutation which is formed naturally when DNA is subjected to oxidative conditions or ionizing radiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that are structural components of alanine transfer RNA. Dihydrouracil and the hydroxy pyrimidines are major base damage products formed by exposure of DNA to ionizing radiation.

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Protocols

Material Safety Data Sheet (Click here)

Glen Report 12.1

Glen Report 23.2

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Notes

5'-Dimethoxytrityl-5-acetoxymethyl-2'-deoxyUridine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C42H51N4O10P M.W.: 802.86 F.W.: 320.19
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 2-3 days
Material Safety Data Sheet

Literature Highlights

Glen Report 12.1: MORE NOVEL MONOMERS

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1093-02 0.25grams .25grams 3.11 90.33 54.2 33.88 24.64 18.07 4.52
10-1093-90 100µmoles .08grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1093-02 0.25grams .25grams 4.65 .067 86.6 54.13 39.36 5.41  
10-1093-90 100µmoles .08grams 1.5 .067 23.6 14.75 10.73 1.48  
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1093-02 0.25grams .25grams 4.65 .067 88.2 55.13 40.09    
10-1093-90 100µmoles .08grams 1.5 .067 25.2 15.75 11.45

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1093-02 0.25grams .25grams 3.11 90.33 54.2 33.88 24.64 18.07 4.52
10-1093-90 100µmoles .08grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1093-02 0.25grams .25grams 4.65 .07 86.6 54.13 39.36 5.41
10-1093-90 100µmoles .08grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1093-02 0.25grams .25grams 4.65 .07 88.2 55.13 40.09

10-1093-90 100µmoles .08grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

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