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5-Hydroxymethyl-dU-CE Phosphoramidite
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Product Specifications
DNA Damage/Repair
Cellular DNA is constantly being damaged by oxidation and alkylation, by free radicals, and by ultraviolet and ionizing radiation. The body has therefore evolved a number of repair enzyme systems to excise and repair these lesions. The 8-oxo purine monomers allow investigation of the structure and activity of oligonucleotides containing an 8-oxo mutation which is formed naturally when DNA is subjected to oxidative conditions or ionizing radiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that are structural components of alanine transfer RNA. Dihydrouracil and the hydroxy pyrimidines are major base damage products formed by exposure of DNA to ionizing radiation.
Usage
- Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
- Deprotection:
Synthesize using acetyl-protected dC (10-1015-xx) and deprotect in 30%
Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 minutes OR
deprotect in Ammonium Hydroxide for 2 hours at room temperature to
remove the acetyl protecting group and complete the deprotection under
temperatures and times suitable to remove the protecting groups of the
nucleobases.
| Specifications |
|---|
| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Refrigerated storage, maximum of 2-8°C, dry |
| Stability In Solution | 2-3 days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions |
|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm |
|---|
| 10-1093-02 | 0.25 g | .25grams | 3.11 | 90.33 | 54.2 | 33.88 | 24.64 | 18.07 | 4.52 |
| 10-1093-90 | 100 µmol | .08grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions |
|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm |
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| 10-1093-02 | 0.25 g | .25grams | 4.65 | 0.07 | 86.6 | 54.13 | 39.36 | 5.41 |
| 10-1093-90 | 100 µmol | .08grams | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
Technical documents
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