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Oligo Synthesis

Oligo Synthesis : Supports, CPGs

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Ac-dC-CPG 500

Ac-dC-CPG 500

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-2013-01Ac-dC-CPG 500 0.1g £11.00 Quantity Add to Order
20-2013-02Ac-dC-CPG 500 0.25g £18.00 Quantity Add to Order
20-2013-10Ac-dC-CPG 500 1.0g £68.00 Quantity Add to Order
20-2113-13Ac-dC-CPG 500 1x10µm £91.00 Quantity Add to Order
20-2113-41Ac-dC-CPG 500 4x1.0µm £55.00 Quantity Add to Order
20-2113-42Ac-dC-CPG 500 4x0.2µm £36.00 Quantity Add to Order
20-2213-41Ac-dC-CPG 500 4x1.0µm £55.00 Quantity Add to Order
20-2213-42Ac-dC-CPG 500 4x0.2µm £36.00 Quantity Add to Order

Description

Ac-dC-CPG 500

Strcture

Catalog Number: 20-2013-xx

(20-2113-xx, 20-2213-xx)

Description: Ac-dC-CPG 500

5'-Dimethoxytrityl-N-Acetyl-2'-deoxyCytidine,
3'-succinoyl-long chain alkylamino-CPG 500
    F.W.: 289.18
Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases.
Storage: Controlled room temperature or lower, dry
Stability in Solution: Not Applicable

STERLING SUPPORTS

All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3’-terminus.

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Protocols

MSDS

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Applications & Benefits

Frequently Asked Technical Question

QUESTION: What is the procedure for deprotecting with AMA?

RESPONSE:The AMA reagent is a 50:50 mixture of aqueous Ammonium

hydroxide and aqueous MethylAmine. With AMA the cleavage of the

oligonucleotide from the support is accomplished in 5 minutes at room

temperature. The deprotection step is carried out at 65°C for a

further 5 minutes. Deprotection can also be carried out at lower

temperatures as shown in Table 1. In all cases, no base modification

has been observed.

TABLE 1: DEPROTECTION WITH AMA

 

Time Temperature
5 min 65°C
10 min 55°C
30 min 37°C
90 min 25°C

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Related products

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