The ExtractNow™ Virus RNA kit is developed for isolating viral RNA from a broad range of starting material such as cell-free biological fluids (plasma, serum, urine, liquor, and cell culture supernatant) but also tissue and swab specimens. Up to 300 μl of fluid and up to 20 mg of solid material can be used. The purified nucleic acids are free of contaminants and suitable for many downstream applications such as PCR and RT-PCR.
PRINCIPLE OF THE METHOD
The method is simple and consists of four general steps: (1) cell lysis, (2) selective binding of nucleic acids to spin columns, (3) removal of residual contaminants and inhibitors, and (4) elution of purified nucleic acids. The procedure does not require phenol/chloroform extraction and needs minimal handling time.
Each kit contains reagents for 10 or 50 extractions. The expiry date of the unopened package is marked on the package label. Store the lyophilized proteinase K at 4 °C. Store the lyophilized Carrier Reagent at –20 °C and all other components at room temperature (18 to 25 °C). Before every use, ensure that all components have reached room temperature. Dissolve any precipitates in the solutions by moderate warming.
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