Cambio - Excellence in Molecular Biology

Cell culture contamination control

Cell culture contamination control: Elimination

Elimination of Mycoplasma contamination

Mynox® Mycoplasma Elimination Reagent

An innovative, rapid and efficient elimination of mycoplasmas in cell cultures and virus stocks. Mynox® is the first biological reagent that eliminates mycoplasmas by killing them, not just inhibiting growth, and is the only anti-mycoplasma agent that can be used to clean virus stocks directly. The cytotoxicity of Mynox is extremely low.

Minerva Biolabs

Catalogue No.DescriptionPack SizePriceQty
10-0200Mynox® Mycoplasma Elimination Reagent2 treatments £95.00 Quantity Add to Order
10-0500Mynox® Mycoplasma Elimination Reagent5 treatments £231.00 Quantity Add to Order
10-1000Mynox® Mycoplasma Elimination Reagent10 treatments £452.00 Quantity Add to Order

Mynox® Mycoplasma Elimination Reagent

An innovative, rapid and efficient elimination of mycoplasmas in cell cultures and virus stocks. Mynox® is the first biological reagent that eliminates mycoplasmas by killing them, not just inhibiting growth, and is the only anti-mycoplasma agent that can be used to clean virus stocks directly. The cytotoxicity of Mynox is extremely low.

Minerva Biolabs

Mynox®

The classic Mynox® is an antibiotic-free formulation; its activity is based on a biophysical mechanism, thus there is no development of resistant strains. Mycoplasma are permanently destroyed within 2-3 hours.

Benefits

Free of antibiotics

Recommended Use / Scope

Mynox® is intended for research use only. Not applicable for clinical treatment. The direct treatment of enveloped viruses is not possible.

Kit Components

Mynox® is a sterile, ready-to-use solution, aliquoted per tube for single use, 200 µl per vial.

Package Sizes

1 application can be used to cure one cell culture permanently from mycoplasma burden.

  • Cat. No. 10-0200 2 applications
  • Cat. No. 10-0500 5 applications
  • Cat. No. 10-1000 10 applications

Result Evaluation

Elimination success should be verified with a suitable mycoplasma detection system, e.g. Minerva Biolabs’s Venor®GeM Mycoplasma PCR Detection Kits.

Required Consumables

6-well tissue culture plate or small tissue culture petri dish (6 mm Ø)
Regular cell culture plastics

Required Lab Devices

Regular cell culture equipment (incubator, centrifuge, pipettes, etc.)

Shelf Life and Storage

Kit components are maintainable at 2 to 8 °C for at least 6 months.

Fig. Electron micrograph showing the effects of Mynox® when applied to contaminated cell cultures. (A) Mycoplasma contaminated cell culture before treatment. (B) With one application of Mynox® membranes of all mycoplasma are destroyed while the tissue cell membrane is unaffected. Black arrows indicate mycoplasmas, white arrows indicate parts of a tissue cell.

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Mynox® Mycoplasma Elimination Reagent

An innovative, rapid and efficient elimination of mycoplasmas in cell cultures and virus stocks. Mynox® is the first biological reagent that eliminates mycoplasmas by killing them, not just inhibiting growth, and is the only anti-mycoplasma agent that can be used to clean virus stocks directly. The cytotoxicity of Mynox is extremely low.

Minerva Biolabs

Mynox® Protocol

Quick visual protocol

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Mynox® Mycoplasma Elimination Reagent

An innovative, rapid and efficient elimination of mycoplasmas in cell cultures and virus stocks. Mynox® is the first biological reagent that eliminates mycoplasmas by killing them, not just inhibiting growth, and is the only anti-mycoplasma agent that can be used to clean virus stocks directly. The cytotoxicity of Mynox is extremely low.

Minerva Biolabs

Citations

Bisen P.S.
,
(2014).
Laboratory Protocols in Applied Life Sciences.
CRC Press
.
Lindt T. and Steubing R., (2013). Atlas of Living Cell Cultures,
Wiley
-
Blackwell.
Roberts J.P.,
(2014). Got Mycoplasma? Find Out with These Culture Testing and Cleaning Solutions.
Biocompare
.
Smit
h C.
,
(2005).
Trouble in the hood: culturing difficult cell types
.
Nature Methods
2
, 385
391.
doi:
10.1038/nmeth0505
-
385
.
Wilson K. and Walker J., (2010). Principles and Techniques of Biochemistry and Molecular Biology.
Cambridge University Press.
Zinöcker S.
et al
., (2011).
Mycoplasma
c
onta
mination revisited: m
esenchymal
stromal cells h
arboring
m
ycoplasma hyorhinis
p
otently
inhibit lymphocyte p
roliferation
i
n
v
itro. PLoS One
6(1):e16005. doi:
10.1371/journal.pone.0016005
.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Mynox® Mycoplasma Elimination Reagent

An innovative, rapid and efficient elimination of mycoplasmas in cell cultures and virus stocks. Mynox® is the first biological reagent that eliminates mycoplasmas by killing them, not just inhibiting growth, and is the only anti-mycoplasma agent that can be used to clean virus stocks directly. The cytotoxicity of Mynox is extremely low.

Minerva Biolabs

Applications

  • Mynox® is effective against M. orale, M. hyorhinis, Acholeplasma laidlawii, M. arginini, M. fermentans, and other mycoplasma species usually encountered as contaminants in cell cultures. Mynox® works for and causes no changes to most cell lines and virus types (e.g. Vero, BHK21, GBK, ML, Hep2, 293, CRFK, H9, Molt4, MT-4, Jurkat) as well as virus stocks (e.g. SHV-1, BHV-1, HSV-1, VSV, SFV, FCV, MEV).

Benefits

  • Effective with only one treatment

  • Permanently destroys mycoplasma within one hour

  • Not an antibiotic, thus there are no resistant strains known to date

  • Temperature-stable

  • Comes in a ready-to-use solution

  • No additional dosing is required during treatment

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Mynox® Mycoplasma Elimination Reagent

An innovative, rapid and efficient elimination of mycoplasmas in cell cultures and virus stocks. Mynox® is the first biological reagent that eliminates mycoplasmas by killing them, not just inhibiting growth, and is the only anti-mycoplasma agent that can be used to clean virus stocks directly. The cytotoxicity of Mynox is extremely low.

Minerva Biolabs

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200