| DesthiobiotinTEG Phosphoramidite |
Catalog Number: 10-1952-xx
Description: DesthiobiotinTEG Phosphoramidite
1-Dimethoxytrityloxy-3-O-(N-desthiobiotinyl-3-aminopropyl)-triethyleneglycolyl-
glyceryl-2-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite |
| Formula: C52H78N5O11P |
M.W.: 980.19 |
F.W.: 539.56 |
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
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| Coupling: 12-15 minute coupling time. To maintain label yield, carry out the synthesis DMT-On. |
| Deprotection: No changes needed from standard method recommended by synthesier manufacturer. To maintain label yield, remove the 5'-DMT after ammonium hydroxide deprotection. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
Biotin-dT can replace dT residues within the oligonucleotide sequence. 5’-Biotin phosphoramidite can be added ONLY ONCE to the 5’-terminus of an oligonucleotide. However, the DMT group on the biotin can be used in RP cartridge and HPLC purification techniques. PC Biotin is a photocleavable 5’-biotin phosphoramidite. BiotinTEG CPG and Protected BiotinLC Serinol CPG are designed for the direct synthesis of oligonucleotides containing biotin at the 3’ terminus.
Desthiobiotin is a biotin analogue that exhibits lower binding to biotin-binding proteins such as streptavidin. This biotin analogue is lacking the sulfur group from the molecule and has a dissociation constant (Kd) several orders of magnitude less than biotin/streptavidin. As a result, biomolecules containing desthiobiotin are dissociated from streptavidin simply in the presence of buffered solutions of biotin. We offer desthiobiotinTEG phosphoramidite and the corresponding CPG.
ABI-style vials and columns are supplied unless otherwise requested.
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