In vitro Transcription: mRNA Quality Control
This section covers a range of products that are designed to produce high yields of high quality RNA. Such applications include synthesis of RNA for nucleic acid amplification, gene expression studies, synthesis of modified RNA transcripts that are resistant to RNAse A-type RNases, studying mechanisms of transcription initiation, rapid, maximum-yield synthesis of RNA for studies of RNA structure, processing, ribozymes, RNAi or antisense RNA
Benchtop PAGE mRNA QC is the New LC/MS
Tired of wait times and costs associated with outsourcing your mRNA
for liquid chromatography/mass spectrometry (LC/MS) 5’ capping
efficiency and 3’ tail length analyses? Now you can easily perform
precise quantitative mRNA 5’ capping efficiency and 3’ poly(A) tail
length assessments using standard lab equipment with the EZ-QC™ mRNA Assay Kits.
Discover quantitative fluorescence-based benchtop polyacrylamide gel
electrophoresis (PAGE) assays for accurate and cost-effective QC of mRNA
5’ capping and 3’ poly(A) tail length analysis in a single day.
Accelerate your mRNA research with the EZ-QC™ Kits for a fraction of the
cost of LC/MS.
Benefits:
- Fast benchtop 5’ capping and 3’ tailing QC analyses
- Low-input mRNA (picomoles, not micromoles!)
- Uses common polyacrylamide gel electrophoresis (PAGE) equipment
- Cost-effective – stretch your budget for more experiments
How Our EZ-QC™ mRNA Assays Work
EZ-QC™ mRNA Capping Efficiency Assay Kit
Quantitates total capping efficiency (% capped vs uncapped RNA) of synthesized mRNA
How it works:
- A chimeric RNA-DNA-RNA Targeting Oligonucleotide (user provided) hybridizes to the 5′ end of synthesized mRNA.
- The hybridization complex undergoes RNase H cleavage, releasing capped and uncapped 5′-end fragments.
- Released capped and uncapped 5′-end fragments are resolved using PAGE and quantitated by fluorescence-based gel band analysis.
EZ-QC™ mRNA Cap 1 Efficiency Assay Kit
Quantitates the percentage of Cap 1 vs Cap 0 caps of synthesized mRNA
How it works:
- 5’ ends of a synthesized mRNA sample are labeled fluorescently with cyanine5 hydrazide (Cy5).
- Hydrolysis of the Cy5-labeled mRNA mixture releases Cy5-labeled Cap 1 and Cap 0 caps.
- Size resolution by PAGE and fluorescence-based gel imaging is used to determine percentage of Cap 1 vs
Cap 0.
EZ-QC™ mRNA Poly(A) Tail Length Assay Kit
Quantitates poly(A) tail length of synthesized mRNA
How it works:
- RNase A is used to digest the synthesized mRNA, leaving only intact Poly(A) tails.
- Poly(A) tails are stained with fluorescent dyes (e.g., SYBR Gold
Nucleic Acid Gel Stain (Invitrogen) + provided poly(A) fluorescence
enhancer).
- Poly(A) tail lengths (from 20 to >300 A’s) are resolved using
PAGE with our proprietary Poly(A) 20-mer Ladder and quantitated by
fluorescence-based gel band analysis.
Products