| DyLight DY647 Phosphoramidite |
Catalog Number: 10-5918-xx
Description: DyLight DY647 Phosphoramidite
1,1'-dimethyl-3,3,3'-trimethyl-3'-(5-[(2-cyanoethyl)-(N,N-diisopropyl)-
phosphoramidityl]pentyl)-indodicarbocyanine tosylate
|
| Formula: C47H63N4O5PS |
M.W.: 827.07 |
F.W.: 516.61 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time recommended. The use of UltraMILD monomers are preferred. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). 0.02M Iodine for oxidation is recommended. |
| Deprotection: If UltraMILD reagents were used, deprotect in 0.05M Potassium Carbonate in Methanol for 4 hours at Room Temperature OR for 2 hours at Room Temperature in 30% Ammonium Hydroxide. If standard bases were used, deprotection in Ammonium Hydroxide at Room Temperature for 24-36 hours will give acceptable yields. However, the oligonucleotide will require a reverse phase purification, e.g. PolyPak. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
DYLIGHT™ DYES
DyLight™ dyes, DY547 and DY647, are phosphoramidite alternatives to Cy3 and Cy5. The performance of these two dyes is similar to the equivalent Cy dyes and the absorption and emission spectra are virtually identical. A comparison of the emission or absorbance spectra of oligos shows that the spectra are virtually identical for Cy3 and DY547. Similarly, Cy5 and DY 647 are virtually identical. When oligos containing Dy547 were excited at 488 nm, the quantum yield (QY) was 12% greater than oligos containing Cy3. When oligos containing Dy647 were excited at 580 nm, the QY was 5% greater and the emission was blue-shifted by only 1 nm compared to Cy5.
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