| Fluorous Chemical Phosphorylation Reagent II |
Catalog Number: 10-1904-xx
Description: Fluorous Chemical Phosphorylation Reagent II
[3-(Fluorous-dimethoxytrityloxy)-2,2-dicarboxyethyl]propyl-(2-cyanoethyl)-
(N,N-diisopropyl)-phosphoramidite |
| Formula: C49H54F17N2O9P |
M.W.: 1168.92 |
F.W.: 79.98 |
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: 6 minute coupling time. Omit the capping step after the addition of this reagent! |
| Deprotection: See Technical Bulletin for details (Technical Bulletin). |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
| Please Note: This product is covered by US Patent No.: 5,959,090. Products for Fluorous Affinity Purification of Oligonucleotides: Patent pending, Berry & Associates, Inc. The use of these products is licensed under U.S. Patents 6,673,539, 6,156,896; 5,859,247; and 5,777,121 and one or more pending patents owned or controlled by Fluorous Technologies, Inc. |
FLUORO-PAK™ PURIFICATION
The fluorous affinity purification of oligonucleotides is a quick and simple affinity-based method for the purification of oligonucleotides that relies on the strong interaction of fluorous-tagged oligonucleotides (made with fluorous-tagged phosphoramidites) with the fluorinated adsorbent present in Fluoro-Pak™ columns. Fluorous affinity purification is operationally similar to DMT-on purification using a reverse-phase (RP) adsorbent, e.g., RP cartridge purification, except that it involves a much stronger affinity interaction and is thus able to afford higher selectivities and recoveries, even with long oligos.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200