Applications

Oligo Synthesis : CEPs

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N2-Amino-Modifier C6 dG

Glen Research

Catalogue No.	Description	Pack Size	Price	Qty	Change to: €
10-1529-02	N2-Amino-Modifier C6 dG	0.25g	£1,100.00	Quantity	Add to Order
10-1529-90	N2-Amino-Modifier C6 dG	100µmoles	£450.00	Quantity	Add to Order
10-1529-95	N2-Amino-Modifier C6 dG	50µmoles	£236.00	Quantity	Add to Order

Description

N2-Amino-Modifier C6 dG

Structure

Catalog Number: 10-1529-xx

Description: N2-Amino-Modifier C6 dG

5'-Dimethoxytrityl-N2-[6-(trifluoroacetylamino)-hex-1-yl]-2'-deoxyGuanosine- 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C₄₈H₆₀F₃N₈O₈P	M.W.: 965.01	F.W.: 428.38

Diluent: Anhydrous Acetonitrile

Coupling: N2-Amino-Modifier C6 dG reacts in a manner identical to normal phosphoramidites. To prevent side reactions, synthesize using acetyl-protected dC. See Deprotection for further details.

Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. The TFA protecting group is removed during standard ammonium hydroxide deprotection. A minor side reaction during ammonia deprotection can lead to irreversibly capping 2-5% of the amine. This could be significant if multiple additions of the modifier are made. To prevent the reaction, synthesize using acetyl-protected dC and deprotect in 30% ammonia/40% methylamine 1:1 (AMA) at 65°C for 10 minutes.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 2-3 days

SEQUENCE MODIFIERS

Sequence Modifiers are designed for use in automated synthesis. The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA, Amino-Modifier dC, Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis. Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports. After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labelled or attached to an enzyme. The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide.