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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

T4 Polynucleotide Kinase, Cloned

T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5'-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3'-monophosphates

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
P0503KT4 Polynucleotide Kinase, Cloned10U/µl 3,000U £155.00£155.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

T4 Polynucleotide Kinase, Cloned

T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5'-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3'-monophosphates

BioSearch Tech (Lucigen/Epicentre)

T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5'-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3'-monophosphates. The enzyme also removes the 3'-phosphate from 3'-phosphoryl polynucleotides, deoxyribonucleoside 3'-monophosphates, and deoxyribonucleoside 3', 5'-diphosphates to form a 3'-hydroxyl group.

T4 polynucleotide kinase
 

Unit Definition

Under standard conditions, one unit converts 1nmole of 32P from [g-32P]-ATP into an acid-insoluble form in 30 minutes at 37°C in 33 mM Tris-acetate, pH 7.8, 66 mM potassium acetate, 10mM magnesium acetate, 0.5mM DTT, 1.0mM ATP, and 0.1mg/ml micrococcal nuclease-treated calf thymus DNA as substrate. 

Storage Buffer

50% glycerol containing 50mM Tris-HCl, pH 7.5, 0.1 M NaCl, 0.1mM EDTA, 0.1% Triton® X-100, and 1 mM DTT. 

T4 PNK 10X Reaction Buffer

330mM Tris-acetate, pH 7.8, 660mM potassium acetate, 100mM magnesium acetate, and 5 mM DTT. A 10mM solution of ATP for non-isotopic applications is available separately. 

Quality Control

T4 PNK is tested in 5'-phosphorylation of nucleic acids and is free of detectable nuclease activities.




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T4 Polynucleotide Kinase, Cloned

T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5'-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3'-monophosphates

BioSearch Tech (Lucigen/Epicentre)

Protocols for: T4 Polynucleotide Kinase, Cloned

T4 Polynucleotide Kinase Protocol

(catalogue number P0505H / P0501K / P0503K) 

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

T4 Polynucleotide Kinase, Cloned

T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5'-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3'-monophosphates

BioSearch Tech (Lucigen/Epicentre)

References

 

  1. Sambrook, J. et al. (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York.
  2. Chaconas, G. et al. (1980) Meth. Enzymol. 65, 75.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

T4 Polynucleotide Kinase, Cloned

T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5'-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3'-monophosphates

BioSearch Tech (Lucigen/Epicentre)

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

T4 Polynucleotide Kinase, Cloned

T4 Polynucleotide Kinase (PNK) catalyses the transfer of the g-phosphate from ATP to the 5'-hydroxyl of single- or double-stranded DNA, RNA, and nucleoside 3'-monophosphates

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Labeling of 5´ termini of DNA and RNA with 32P or 33P for DNA sequencing, blot-hybridization experiments, or transcript mapping using Mung Bean Nuclease, S1 nuclease, or other nucleases.1,2
  • Phosphorylation of oligonucleotide linkers and other DNA or RNA molecules prior to ligation, or for use in ligation amplification reactions with Ampligase® Thermostable DNA Ligase.
  • Preparation of labeled DNA or RNA molecular weight markers for gel electrophoresis and chromatography.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200