| Pyrrolo-dC-CE Phosphoramidite |
Catalog Number: 10-1017-xx
Description: Pyrrolo-dC-CE Phosphoramidite
5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-
2'-deoxyribonucleoside, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula: C42H50N5O7P |
M.W.: 767.85 |
F.W.: 327.23 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Standard coupling time. Use 0.02 M Iodine for Oxidation. |
| Deprotection: Ammonium Hydroxide for 24 hours at room temperature. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: This product is covered by US Patent No.: 7,144,995. Patented under European patent number 1483280. |
FLUORESCENT NUCLEOSIDES
Etheno-dA is a fluorescent nucleoside which is especially useful in observing the transition between DNA structural types. It is quite base labile and should be deprotected with ammonium hydroxide at room temperature for 24 hours. Alternatively, UltraMild chemistry can be used. 2-Aminopurine and AP-dC (G-Clamp) are also useful fluorescent nucleosides.
Pyrrolo-dC is a fluorescent deoxycytidine analog that is an ideal probe of DNA structure and dynamics. It base-pairs as a normal dC nucleotide. An oligo fully substituted with pyrrolo-dC has the same ™ as the control dC oligo with the same specificity for dG. Its small size does not perturb the structure of the DNA helix and it is well tolerated by a number of DNA and RNA polymerases. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-dCTP has potential uses in biological assay development.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200