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Aptamers

Aptamers: Catalogue Aptamers

Important information

Do you know what modifications you will need for your aptamers?  see "modifications" section for details

PBP-2a (MRSA) Aptamer to Bacterial Protein

MRSA PBP-2a Aptamer ATW0032

Base Pair Bio

Catalogue No.DescriptionPack SizePriceQty
ATW0032-100PBP-2a (MRSA) 10 AEU (100ug) POA Quantity Add to Order
ATW0032-25PBP-2a (MRSA) 2.5 AEU (25ug) POA Quantity Add to Order

Description

Methicillin-resistant Staphylococcus aureus, abbreviated MRSA, is one of a number of greatly feared strains of S. aureus which have become resistant to most B lactam antibiotics. Resistance is conferred by the mecA gene, which codes for an altered penicillin-binding protein (PBP2a or PBP2') that has a lower affinity for binding B lactams (penicillins, cephalosporins, and carbapenems). This allows for resistance to all B lactam antibiotics, and obviates their clinical use during MRSA infections.

This aptamer was selected by scientists at Base Pair Biotechnologies using proprietary methods and Recombinant Staphylococcus aureus PBP2a, [Ray Biotech Cat # 230-00051] as a target and 1X PBS, pH 7.4, 0.01% BSA, 0.002% Tween20.  Let us know if you have any questions about its development or potential uses.

Target:   PBP-2a, Penicillin-binding-protein 2a from Staphylococcus aureus

Target Species:   bacterial

Target Type:   protein

Affinity Constant (Kd):   2 nM

Default 3' Linker (Spacer) is 10T. Please submit a custom quote request for other options.

Aptamer information Kd 1.96± 0.20 nM

MRSA ATW0032 #819 datasheet

ELISA data ATW0032

Please choose your modification and add as an extra catalogue number:
ATWUNMOD - Unmodified
ATWAMINE - Amine modification
ATWBIOTIN - Biotin modification
ATWTHIOL - Thiol modification (extra charge)
ATWFLUOR - Fluor (all) modification (extra charge)


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Protocols

MRSA ATW0032 #819 datasheet

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Notes

Antibody Equivalent Units (AEU). There is a  large molecular weight difference in antibodies (typically 150,000 Da) and Base Pair’s aptamers (~14,000 Da). We provide our aptamers in terms of moles, where the aptamer moles in 1  AEU is equal to antibody moles in the typical 100 microgram shipment from a leading antibody vendor. Therefore our standard 2.5 AEU (25ug) or 5 AEU (50ug) delivery is 2.5 and 5 times the equivalent moles of a standard antibody delivery of 100 microgram respectively

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200