Description
The AllspinTM total DNA/RNA purification kit provides a convenient method for the isolation of total DNA and total RNA simultaneously from a single sample of tissue or cultured cells. DNA and RNA are purified separately from a same sample by individual but a successive procedure using column B and column W respectively. Whole procedure can be performed in just 30 minutes. The maximum length DNA obtainable is 50 kb (average 30 kb) and that of RNA is longer than 200 nucleotides.
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Features and Benefits
Glass fibre membrane technology
Sample size : Up to 20 mg tissue or up to 1 x 107 cultured cells
Typical yield of RNA : Up to 20ug / 1 x 106 cultured cells Up to 60 ug / 10 mg liver tissue
Typical yield of DNA : Up to 10 ug / 1 x 106 cultured cells Up to 25 ug / 10 mg liver tissue
High purity
Preparation time : ~ 30 minutes
Stable and consistent yield
No phenol / chloroform extraction
No ethanol precipitation
Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular Cloning, real-time PCR, RNase protection assays and other analytical procedures
Click here for protocol
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