Catalog Number: 20-2975-xx
Description: 3'-Cholesteryl-TEG CPG
1-Dimethoxytrityloxy-3-O-(N-cholesteryl-3-aminopropyl)-triethyleneglycol-
glyceryl-2-O-succinoyl-long chain alkylamino-CPG |
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F.W.: 755.97 |
Diluent: Not Applicable |
| Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. |
| Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: Not Applicable |
CHOLESTEROL LABELLING
Potential therapeutic oligonucleotides must permeate the cell membrane for optimal activity. The addition of lipophilic groups to an oligonucleotide would be expected to enhance activity. The use of cholesteryl oligos and the consequent improvement in activity has been described. We have designed our Cholesteryl Phosphoramidite with our branched triethyleneglycol (TEG) spacer for maximum solubility in acetonitrile as well as for applications requiring multiple labels.
ABI-style vials and columns are supplied unless otherwise requested (see note box).
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