Catalog Number: 20-5912-xx
Description: 3'-Dabcyl CPG
1-Dimethoxytrityloxy-3-[O-(N-4'-carboxy-4-(dimethylamino)-
azobenzene)-3-aminopropyl)]-propyl-2-O-succinoyl-long chain alkylamino-CPG |
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F.W.: 462.44 |
Diluent: Not Applicable |
| Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. |
| Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. |
| Storage: Freezer storage, -10 to -30°C, dry. Light sensitive material. |
| Stability in Solution: Not Applicable |
Dabcyl LABELLING
A molecular beacon probe1 has its natural fluorescence quenched in solution unless it is hybridized to the target sequence. Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer. The Dabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and 3’-Dabcyl CPG are used to prepare probes with the quencher blocking the 3’-terminus. 5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminus and Dabcyl-dT places it within the sequence, leaving the 3’-terminus available for polymerase extension.
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