| 5'-Dabcyl Phosphoramidite |
Catalog Number: 10-5912-xx
Description: 5'-Dabcyl Phosphoramidite
6-(N-4'-carboxy-4-(dimethylamino)azobenzene)-aminohexyl-
1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite |
| Formula: C30H45N6O3P |
M.W.: 568.69 |
F.W.: 430.18 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time recommended |
| Deprotection: No changes needed from standard methodrecommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
Dabcyl LABELLING
A molecular beacon probe1 has its natural fluorescence quenched in solution unless it is hybridized to the target sequence. Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer. The Dabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and 3’-Dabcyl CPG are used to prepare probes with the quencher blocking the 3’-terminus. 5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminus and Dabcyl-dT places it within the sequence, leaving the 3’-terminus available for polymerase extension.
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