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Oligo Synthesis

Oligo Synthesis : RNA synthesis

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A-TOM-CE Phosphoramidite

A-TOM-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3004-02A-TOM-CE Phosphoramidite 0.25g £68.00 Quantity Add to Order
10-3004-05A-TOM-CE Phosphoramidite 0.5g £136.00 Quantity Add to Order
10-3004-10A-TOM-CE Phosphoramidite 1.0g £250.00 Quantity Add to Order

Description

A-TOM-CE Phosphoramidite

Structure

Catalog Number: 10-3004-xx

Description: A-TOM-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-Adenosine,2'-O-triisopropylsilyloxymethyl-
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C52H72N7O9PSi M.W.: 998.24 F.W.: 329.21
Diluent: Anhydrous Acetonitrile
Coupling: See Technical Bulletin
Deprotection: See Technical Bulletin for details (www.glenresearch.com/Technical/TB_RNA_TOM_Deprotection.pdf). Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours
Please Note: TOM-RNA Phosphoramidites are supplied under agreement with QIAGEN. RNA synthesis using the TOM-Protecting-Group is covered by US Patent No. 5,986,084.

TOM-Protected RNA Phosphoramidites

RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2’ to 3’ migration. This migration under basic conditions leads to non-biologically active 2’-5’ linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2’-O-TBDMS protection.

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Applications & Benefits

IMPROVEMENT IN RNA SYNTHESIS

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3004-02 0.25grams .25grams 2.5 70 42 26.25 19.09 14 3.5
10-3004-05 0.5grams .5grams 5.01 153.67 92.2 57.63 41.91 30.73 7.68
10-3004-10 1.0gram 1grams 10.02 320.67 192.4 120.25 87.45 64.13 16.03
10-3004-2S 20grams 20grams 200.35 6665 3999 2499.38 1817.73 1333 333.25
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3004-02 0.25grams .25grams 3.74 .07 N/A N/A 14.25 N/A
10-3004-05 0.5grams .5grams 7.48 .07 N/A N/A 29.83 N/A
10-3004-10 1.0gram 1grams 14.95 .07 N/A N/A 60.96 N/A
10-3004-2S 20grams 20grams 299.03 .07 N/A N/A 1244.63 N/A
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3004-02 0.25grams .25grams 3.74 .07 N/A 11.29 10.61

10-3004-05 0.5grams .5grams 7.48 .07 N/A 23.35 21.94

10-3004-10 1.0gram 1grams 14.95 .07 N/A 47.45 44.58

10-3004-2S 20grams 20grams 299.03 .07 N/A 963.84 905.42

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Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200