We offer a range of EZ Biosystems' general purpose transfection reagents for plasmid, siRNA, co-transfections, in vivo work and CRISPR. These reagents are cost-effective and have been specially formulated for maximum transfection efficiency of a wide range of different cell types with minimal toxicity.
Avalanche® transfection reagents have the broad spectrum for
the transfection of large plasmid, mRNA, siRNA, and/or other type of
nucleic acids. This feature makes Avalanche® transfection reagents the
best option for co-transfection of different type and/or size of nucleic
acids. The following are the reasons for the broad spectrum:
The
key components of Avalanche® transfection reagents are mixtures of
cationic lipids and cationic polymers, which contain all three types of
amines: primary, secondary, and tertiary amines. The three types of
amines have different positive charge strength, hence have different
capabilities to combine with negative charged anions.
As anions, different types and sizes of nucleic acids have different densities and quantities of negative charges.
With
optimized ratios of different cationic lipids and polymers, hence
optimized ratios of the three different type of amines, together with
optimized protocols, our Avalanche® mixtures have the optimized strength
of positive charges that ensure them to efficiently combine with
different type of nucleic acids of a variety of negative charge
densities and quantities, and compact them into the right sizes of
nanoparticles with the right densities of positive charge (zeta
potential) on their surface. The optimized size and the right strength
of zeta potential of the nanoparticles are the keys for the
nanoparticles to interact efficiently with negatively charged cell
surface, and trigger endocytosis process.
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